Flow Cytometry Core Publications

How to Measure “Spillover Spread.”

Pyruvate Modulation of Redox Potential Controls Mouse Sperm Motility.

Side-by-Side Comparison of Compensation Beads Used in Polychromatic Flow Cytometry

Retinoic Acid is Dispensable for Meiotic Initiation But Required for Spermiogenesis in the Mammalian Testis.

  • Kirsanov O, Johnson TA, Niedenberger BA, Malachowski TN, Hale BJ, Chin Q, Lackford B, Wang J, Singh A, Schindler K, Hermann BP, Hu G, Geyer CB.
  • Development. 2023 dev.201638 doi: https://doi.org/10.1242/dev.201638
  • Open Access Article.

Simultaneous Inhibition of Ceramide Hydolysis and Glycosylation Synergizes to Corrupt Mitochondrial Respiration and Signal Caspase Driven Cell Death in Drug-Resistant Acute Myeloid Leukemia.

  • Fisher-Wellman KH, Kassai M, Hagen JT, Neufer PD, Kester M, Loughran TP Jr., Chalfant CE, Feith DJ, Tan S-F, Fox TE, Ung J, Fabrias G, Abad JL, Sharma A, Golla U, Claxton DF, Shaw JJP, Bhowmick D, Cabot MC.
  • Cancers. 2023; 15(6):1883. https://doi.org/10.3390/cancers15061883
  • Open Access Article.

Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.

  • Kirsanov O, Johnson T, Malachowski T, Niedenberger BA, Gilbert EA, Bhowmick D, Ozdinler PH, Gray DA, Fisher-Wellman K, Hermann BP, Geyer CB.
  • Development. 2022 Oct 17:dev.200713. doi: 10.1242/dev.200713.
  • PMID: 36250451
  • Lempicki M, Paul S, Serbulea V, Upchurch CM, Sahu S, Gray JA, Ailawadi G, Garcia BL, McNamara CA, Leitinger N, Meher AK. 
  • Cell Immunol. 2022 Sep 9;381:104603. doi: 10.1016/j.cellimm.2022.104603.
  • PMID: 36182705
A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry.
  • Bhowmick D, van Diepen F, Pfauth A, Tissier R, Ratliff M.
  • Sci Rep. 2021 Oct;11:20553. doi: 10.1038/s41598-021-99831-7. Epub 2021 Oct 15.
  • PMID: 34654870 Free PMC article.

Tolerogenic vaccines: Targeting the antigenic and cytokine niches of FOXP3+ regulatory T cells.

Differential RA responsiveness directs formation of functionally distinct spermatogonial populations at the initiation of spermatogenesis in the mouse.

  • Velte EK, Niedenberger BA, Serra ND, Singh A, Roa-DeLaCruz L, Hermann BP, Geyer CB.
  • Development. 2019 May 13;146(12):dev173088. doi: 10.1242/dev.173088.
  • PMID: 31023878 Free PMC article.
A GM-CSF-neuroantigen tolerogenic vaccine elicits inefficient antigen recognition events below the CD40L triggering threshold to expand CD4+ CD25+ FOXP3+ Tregs that inhibit experimental autoimmune encephalomyelitis (EAE).
Low-Zone IL-2 Signaling: Fusion Proteins Containing Linked CD25 and IL-2 Domains Sustain Tolerogenic Vaccination in vivo and Promote Dominance of FOXP3+ Tregs in vitro.
  • DeOca KB, Moorman CD, Garcia BL, Mannie MD.
  • Front Immunol. 2020 Sep 23;11:541619. doi: 10.3389/fimmu.2020.541619. eCollection 2020.
  • PMID: 33072087 Free PMC article.
A GMCSF-Neuroantigen Tolerogenic Vaccine Elicits Systemic Lymphocytosis of CD4+ CD25high FOXP3+ Regulatory T Cells in Myelin-Specific TCR Transgenic Mice Contingent Upon Low-Efficiency T Cell Antigen Receptor Recognition.
  • Moorman CD, Curtis AD 2nd, Bastian AG, Elliott SE, Mannie MD.
  • Front Immunol. 2019 Jan 10;9:3119. doi: 10.3389/fimmu.2018.03119. eCollection 2018.
  • PMID: 30687323 Free PMC article.