Materials Needed

ReagentVendorCat noUse
FACS BufferBD554657For surface stain and washing
Biolegend420201
Annexin V BufferBD556454Annexin V only binds in this buffer
Biolegend422201
ThermoV13246
Annexin V-FITCBiolegend640906Detecting Apoptosis
DAPIBiolegend422801Cell cycle, Live Dead dye
Propidium iodideSigmaP4170-25MG
DRAQ5™Biolegend424101DNA stain for both live and fixed cell
EDU kit-Alexa 488ThermoC10337Cell Cycle kinetics
Human BD Fc BlockBD564219Blocking binding of CD16/32 antigens to the FC region of Ab
Human TruStain FcX™ (Fc Receptor Blocking Solution)Biolegend422302
Mouse BD Fc BlockBD553141
TruStain FcX™ (anti-mouse CD16/32) AntibodyBiolegend101320
Mouse serumThermo10410Blocking Non-Specific Ab binding
Goat serum31872
Rabbit serumJan-01
True-Stain Monocyte BlockerBiolegend426102Blocks NSB of Cyanine dyes to Monocyte
FOXP3 Fix/Perm Buffer Set421403intracellular staining FOXP3 with minimum effect on the surface fluorochrome, except Tandems
True-Phos™ Perm Buffer425401This buffer has been designed for use with antibodies for phospho-proteins (e.g. p-STAT6, p-ERK, etc.) and other intracellular targets (e.g. Cyclin B1, pan-STAT3, etc.)
True-Nuclear™ Transcription Factor Buffer Set424401Intracellular staining with minimum effect on the surface fluorochrome staining
Fixation Buffer420801Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins.
Intracellular Staining Permeabilization Wash Buffer (10X)421002 used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer
Formaldehyde 4% stabilised, buffered (pH 7.0 ±0.1)VWRVWRC9713.1000Fixing cells, 0.05% for 30 min
Foxp3 / Transcription Factor Fixation/PermeabilizationeBioscience00-5521-00for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67
BD Cytofix/Cytoperm™ Fixation/Permeabilization KitBD554714This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines
Brilliant stan buffer563794Use when more than one brilliant dye is present
FACS Tube NO CAPCorning352052Cell harvest/stain/wash/loading
FACS Tube with CAP352054
FACS Tube Polypropylene352063
FACS tube with strainer352235Filtering cell aggregates
Cell strainer capStellar ScientificFSCFLTCP
Anti-Mouse Ig, κ/Negative Control Compensation Particles SetBD552843These beads have ability to bind to Abs. This will produce a very bright signal which used for compensation or unmixing when cells are not available.
BD™ CompBead Plus Anti-Mouse Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set60497
BD™ CompBeads Anti-Rat Ig, κ/Negative Control Compensation Particles Set552844
BD™ CompBead Plus Anti-Rat Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set560499
BD™ CompBeads Anti-Rat and Anti-Hamster Ig κ /Negative Control Compensation Particles Set552845
UltraComp eBeads™ Compensation BeadsThermo Fisher Scientific01-2222-42
OneComp eBeads™ Compensation Beads01-1111-42
AbC™ Total Antibody Compensation BeadA10513
MACS® Comp Bead Kit, anti-mouse IgκMiltenyi130-097-900
VersaComp Antibody Capture KitBeckman CoulterB22804
SpectraComp® Compensation BeadsSlingshotSSB-05-A
COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated ParticlesSpherotechCMigp-30-5H
Compensation beadBiolegend424601
10X RBC Lysis Buffer (Multi-species)Thermo Fisher Scientific00-4300-54Buffer containing NH4Cl to lyse RBC but keep leukocytes live
RBC Lysis Buffer (10X)Biolegend420301
RBC Lysis Buffer (10X)Cell Signaling Technology46232
Pharm Lyse™BD555899
FACS™ Lysing Solution349202Buffer containing <10% formaldehyde and <50% diethylene glycol to lyse the RBC and fix the leukocytes
OptiLyse® C Lysis SolutionBeckmanA11895Buffer containing 1.5% formaldehyde to lyse the RBC and fix the leukocytes