FACS Buffer | BD | 554657 | For surface stain and washing | |
Biolegend | 420201 | |
Annexin V Buffer | BD | 556454 | Annexin V only binds in this buffer | |
Biolegend | 422201 | |
Thermo | V13246 | |
Annexin V-FITC | Biolegend | 640906 | Detecting Apoptosis | |
DAPI | Biolegend | 422801 | Cell cycle, Live Dead dye | |
Propidium iodide | Sigma | P4170-25MG | |
DRAQ5™ | Biolegend | 424101 | DNA stain for both live and fixed cell | |
EDU kit-Alexa 488 | Thermo | C10337 | Cell Cycle kinetics | |
Human BD Fc Block | BD | 564219 | Blocking binding of CD16/32 antigens to the FC region of Ab | |
Human TruStain FcX™ (Fc Receptor Blocking Solution) | Biolegend | 422302 | |
Mouse BD Fc Block | BD | 553141 | |
TruStain FcX™ (anti-mouse CD16/32) Antibody | Biolegend | 101320 | |
Mouse serum | Thermo | 10410 | Blocking Non-Specific Ab binding | |
Goat serum | 31872 | |
Rabbit serum | Jan-01 | |
True-Stain Monocyte Blocker | Biolegend | 426102 | Blocks NSB of Cyanine dyes to Monocyte | |
FOXP3 Fix/Perm Buffer Set | 421403 | intracellular staining FOXP3 with minimum effect on the surface fluorochrome, except Tandems | |
True-Phos™ Perm Buffer | 425401 | This buffer has been designed for use with antibodies for phospho-proteins (e.g. p-STAT6, p-ERK, etc.) and other intracellular targets (e.g. Cyclin B1, pan-STAT3, etc.) | |
True-Nuclear™ Transcription Factor Buffer Set | 424401 | Intracellular staining with minimum effect on the surface fluorochrome staining | |
Fixation Buffer | 420801 | Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. | |
Intracellular Staining Permeabilization Wash Buffer (10X) | 421002 | used to permeabilize cells following fixation with Intracellular Staining Fixation Buffer | |
Formaldehyde 4% stabilised, buffered (pH 7.0 ±0.1) | VWR | VWRC9713.1000 | Fixing cells, 0.05% for 30 min | |
Foxp3 / Transcription Factor Fixation/Permeabilization | eBioscience | 00-5521-00 | for staining with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67 | |
BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit | BD | 554714 | This kit enables the fixation and permeabilization of cells which is necessary for staining intracellular cytokines | |
Brilliant stan buffer | 563794 | Use when more than one brilliant dye is present | |
FACS Tube NO CAP | Corning | 352052 | Cell harvest/stain/wash/loading | |
FACS Tube with CAP | 352054 | |
FACS Tube Polypropylene | 352063 | |
FACS tube with strainer | 352235 | Filtering cell aggregates | |
Cell strainer cap | Stellar Scientific | FSCFLTCP | |
Anti-Mouse Ig, κ/Negative Control Compensation Particles Set | BD | 552843 | These beads have ability to bind to Abs. This will produce a very bright signal which used for compensation or unmixing when cells are not available. | |
BD™ CompBead Plus Anti-Mouse Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set | 60497 | |
BD™ CompBeads Anti-Rat Ig, κ/Negative Control Compensation Particles Set | 552844 | |
BD™ CompBead Plus Anti-Rat Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set | 560499 | |
BD™ CompBeads Anti-Rat and Anti-Hamster Ig κ /Negative Control Compensation Particles Set | 552845 | |
UltraComp eBeads™ Compensation Beads | Thermo Fisher Scientific | 01-2222-42 | |
OneComp eBeads™ Compensation Beads | 01-1111-42 | |
AbC™ Total Antibody Compensation Bead | A10513 | |
MACS® Comp Bead Kit, anti-mouse Igκ | Miltenyi | 130-097-900 | |
VersaComp Antibody Capture Kit | Beckman Coulter | B22804 | |
SpectraComp® Compensation Beads | Slingshot | SSB-05-A | |
COMPtrol Kit, Goat anti-Mouse Ig (H&L) Coated Particles | Spherotech | CMigp-30-5H | |
Compensation bead | Biolegend | 424601 | |
10X RBC Lysis Buffer (Multi-species) | Thermo Fisher Scientific | 00-4300-54 | Buffer containing NH4Cl to lyse RBC but keep leukocytes live | |
RBC Lysis Buffer (10X) | Biolegend | 420301 | |
RBC Lysis Buffer (10X) | Cell Signaling Technology | 46232 | |
Pharm Lyse™ | BD | 555899 | |
FACS™ Lysing Solution | 349202 | Buffer containing <10% formaldehyde and <50% diethylene glycol to lyse the RBC and fix the leukocytes | |
OptiLyse® C Lysis Solution | Beckman | A11895 | Buffer containing 1.5% formaldehyde to lyse the RBC and fix the leukocytes | |