Zeiss LSM 900 Confocal

Zeiss LSM 900 Confocal is located in Brody 3S-08.

Please contact Dr. Debajit Bhowmick to arrange for training.

The LSM 900 system features outstanding sensitivity, suitable for classical confocal microscopy and special applications, such as live cell and spectral imaging. This inverted scope is designed for complex tasks while being easy to operate.

For many users it will provide an entry to high-end applications, with frame rates of up to five fps (512 x 512 pixels), a freely 360° rotatable scanning field and freely definable regions of interest (ROIs).

The LSM 900 features:

• 3D imaging
• Multiple fluorescence and colocalization analyses
• Emission Fingerprinting
• Live Cell Imaging (onstage incubator)
• FRAP, FLIP, photoactivation and photoconversion

Laser lines –  405nm, 488nm, 555nm, and 639nm.

Double Bandpass Filters:
BP 420-475  +  BP  500-610 (for 405/488 laser lines)
BP  555-610  +  LP650 ( for 555/639 laser lines)

Objectives  (Magnification/Numerical Aperture):

• 10X/0.3  Air  ECPlan -Neofluar
• 20X/0.8  Air  Plan Apochromat
• 40X/1.4  Oil  Plan Apochromat
• 63X/1.2  Water  Plan Apochromat
• 100X/1.3 Oil  Plan Neofluar

For more information or protocols click on the “LSM 700 Resources” tab.

The LSM 700 was acquired in 2013 through funds made available from the North Carolina Biotechnology Center’s Institutional Development Grant and the BSOM Office of Research and Graduate Studies.