LI-COR Biosciences Odyssey® Imaging System

The Odyssey Imaging System offers exceptional versatility for protein and other target imaging compared to other available systems. This system uses infrared and near-infrared fluorescent probes that possess a very wide linear dynamic range with two channels so that two separate targets can be visualized and quantified simultaneously. The following description provides additional explanations of the advantages that this system provides for performing protein and other target analysis.

1. Wide Linear Range – Through the use of fluorescently labeled antibodies rather than an enzymatic reaction, the Odyssey provides a broad linear dynamic range to accurately detect strong and weak bands on the same Western blot.

2. High Sensitivity – The use of the infrared spectrum provides for equal to or better sensitivity than chemiluminescence because autofluorescence and light scatter is dramatically reduced. This results in the cleanest background, highest signal-to-noise ratios, and best detection sensitivity available with a fluorescent system.

3. Direct Detection – When using the Odyssey system, there is no film, darkroom, or messy substrates. The dyes are stable on the membranes for years (as long as they are properly stored).

4. Flexible System with Many Applications

Applications include:

  • Coomassie Gels
  • DNA Gel Staining
  • ELISA/FLISA
  • EMSA/Gel Shift Assay
  • Glycoprotein Detection
  • In-Cell Western Assay
  • In-Gel Western Assay
  • Northern Blot
  • Protein Array
  • Quantitative Western Blot
  • Reporter Gene Assay
  • Reverse Phase Array
  • RNAi Screens
  • Small Animal Imaging (uses the MousePod but also see Pearl Imager)
  • Southern Blot
  • Tissue Section Imaging
  • Transcription Factor Assay

5. Multiple User Capability – The software provided for acquisition and analysis is site licensed at no cost and with the extended warranty lifetime upgrades are provided at no cost.

The instrument is currently housed in Rm. 6S11 in the Brody Medical Sciences building. If you are interested in information regarding this item of equipment and its use, please contact Dr. David A. Taylor or Ms. Kathleen Thayne in the Department of Pharmacology and Toxicology.